Glutathione Reductase Assay Kit (BA0087)
Glutathione Reductase Assay Kit - InformationAssay Genie's non-radioactive, colorimetric Glutathione Reductase Assay Kit is designed to accurately measure glutathione reductase (GR) activity in biological samples with a method that utilizes Ellman's method in which DTNB reacts with the GSH generated from the reduction of GSSG by glutathione reductase in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione reductase activity in the sample.
ApplicationsFor quantitative determination of glutathione reductase activity.
Glutathione Reductase Assay Kit - Key Features
- Fast and sensitive. Linear detection range (20 sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Glutathione Reductase Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL GDH: 120 uL Substrate: 1 mL DTNB: 60 uL Cosubstrate: 1 mL Calibrator: 1.5 mL|
|Kit Requires||Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader|
|Method of Detection||OD412nm|
|Detection Limit||0.4 U/L|
|Samples||Plasma, serum, tissue and culture media etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C upon receiving|
|Shelf Life||6 months|
More DetailsGLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. Assay Genie' non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman's method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample