The Glutathione Synthetase (GSS) Monoclonal Antibody (CAB11557) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high specificity and sensitivity for detecting the glutathione synthetase protein in various samples. Validated for use in Western blot applications, the antibody provides accurate and reliable results for analyzing glutathione synthetase levels in different cell types.Glutathione is a critical antioxidant that plays a key role in protecting cells from oxidative stress and maintaining overall cellular function.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Glutathione Synthetase (GSS) Monoclonal Antibody
SKU:
CAB11557
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0630
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
HeLa, 293T, HepG2, Mouse lung, Mouse liver, Mouse kidney, Rat lung, Rat liver, Rat kidney
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
52kDa
Observed MW:
52kDa
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Purification Method
Affinity purification
Gene ID
2937
RRID
AB_2861596
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Glutathione Synthetase (GSS) (GSS) Rabbit mAb (CAB11557) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunofluorescence analysis of NIH-3T3 cells using Glutathione Synthetase (GSS) (GSS) Rabbit mAb (CAB11557) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
