Anti-GRIA3 Antibody (CAB1159)

Antibody Name:	Anti-GRIA3 Antibody
Antibody SKU:	CAB1159
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-490 of human GRIA3 (NP_000819.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-251MG, PC-3, Mouse brain, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 200-490 of human GRIA3 (NP_000819.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QEFR RIIE EMDR RQEK RYLI DCEV ERIN TILE QVVI LGKH SRGY HYML ANLG FTDI LLER VMHG GANI TGFQ IVNN ENPM VQQF IQRW VRLD EREF PEAK NAPL KYTS ALTH DAIL VIAE AFRY LRRQ RVDV SRRG SAGD CLAN PAVP WSQG IDIE RALK MVQV QGMT GNIQ FDTY GRRT NYTI DVYE MKVS GSRK AGYW NEYE RFVP FSDQ QISN DSAS SENR TIVV TTIL ESPY VMYK KNHE QLEG NERY EGYC VDLA YEIA KHVR IKYK LSIV GDGK YGAR DPET KIW
Gene ID:	2892
Uniprot:	P42263
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:	101kDa
Observed MW:	101kDa

Synonyms:

GRIA3, GLUR-C, GLUR-K3, GLUR3, GLURC, GluA3, MRX94

Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties.

UniProt Protein Function:	GluR3: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Interacts with protein kinase C-alpha binding protein. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated Chromosomal Location of Human Ortholog: Xq25 Cellular Component: postsynaptic membrane; dendrite; plasma membrane; cell junction Molecular Function:extracellular-glutamate-gated ion channel activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Biological Process: synaptic transmission, glutamatergic; synaptic transmission; transport; glutamate signaling pathway; ionotropic glutamate receptor signaling pathway Disease: Mental Retardation, X-linked, Syndromic, Wu Type
NCBI Summary:	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]
UniProt Code:	P42263
NCBI GenInfo Identifier:	77416864
NCBI Gene ID:	2892
NCBI Accession:	P42263.2
UniProt Secondary Accession:	P42263,Q4VXD5, Q4VXD6, Q9HDA0, Q9HDA1, Q9HDA2, Q9P0H1 D3DTF1,
UniProt Related Accession:	P42263
Molecular Weight:	101,228 Da
NCBI Full Name:	Glutamate receptor 3
NCBI Synonym Full Names:	glutamate receptor, ionotropic, AMPA 3
NCBI Official Symbol:	GRIA3
NCBI Official Synonym Symbols:	GLUR3; GLURC; GluA3; MRX94; GLUR-C; GLUR-K3
NCBI Protein Information:	glutamate receptor 3; gluR-3; dJ1171F9.1; glutamate receptor C; glutamate receptor subunit 3; AMPA-selective glutamate receptor 3; glutamate receptor, ionotrophic, AMPA 3
UniProt Protein Name:	Glutamate receptor 3
UniProt Synonym Protein Names:	AMPA-selective glutamate receptor 3; GluR-C; GluR-K3; Glutamate receptor ionotropic, AMPA 3; GluA3
Protein Family:	Glutamate receptor
UniProt Gene Name:	GRIA3
UniProt Entry Name:	GRIA3_HUMAN

	Western blot analysis of extracts of various cell lines, using GRIA3 antibody (CAB1159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using GRIA3 antibody (CAB1159). Blue: DAPI for nuclear staining.

Antibodies

Anti-GRIA3 Antibody (CAB13993)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)