The Glutathione (GSH) ELISA Kit is a specialized assay designed for the accurate measurement of glutathione levels in biological samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.Glutathione is a powerful antioxidant that plays a critical role in protecting cells from oxidative damage and maintaining cellular health. Imbalances in glutathione levels have been linked to various diseases such as cancer, liver disease, and neurodegenerative disorders, highlighting its importance as a potential biomarker for understanding and treating these conditions. With its advanced technology and ease of use, the GSH ELISA Kit is a valuable tool for researchers seeking to investigate the role of glutathione in health and disease, as well as for developing new therapeutic strategies targeted at restoring glutathione homeostasis. Order your kit today and unlock the potential of glutathione research in your laboratory.
Product Name:
GSH (Glutathione) ELISA Kit
SKU:
UNES00017
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 μg/mL
Detection range:
1.56-100 μg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
