The HRP-conjugated Goat anti-Mouse IgG2a (CABS065) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins. RRID AB_2769853 Gene ID Swiss Prot Synonym
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
HRP-conjugated Goat anti-Mouse IgG2a
SKU:
CABS065
Size:
100μL, 200μL
Reactivity:
Mouse
Clone Number:
-
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:5000
ELISA
1:2000 - 1:5000
Synonyms:
-
Positive Sample:
Mouse IgG2a
Cellular Localization:
-
Calculated MW:
-
Observed MW:
25kDa(Light chain),55kDa(Heavy chain)
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins. RRID AB_2769853 Gene ID Swiss Prot Synonym
Purification Method:
Affinity purification
Gene ID:
-
RRID:
AB_2769853
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 50% glycerol,pH7.4.
Western blot analysis of lysates from Mouse IgG using HRP Goat Anti-Mouse IgG2a (CABS065) at 1:2000 dilution. Lysates/proteins: 100ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
