HSP22 Antibody is a rabbit polyclonal antibody directed against HSP22, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF and IP. Reported reactivity: Human, Mouse and Rat. Purified by Peptide Affinity.
HSP22, also known as HSPB8, is a 196-amino acid member of the small heat shock protein (sHSP) superfamily, closely related to HSP27. While predominantly expressed in skeletal muscle, heart, and placenta, HSP22 is increasingly recognized for its critical role in neuronal health and neurodegenerative disease. Functioning as a monomer, HSP22 exhibits temperature-dependent chaperone activity and interacts with other sHSPs, including HSPB1 (HSP27). It preferentially binds phosphorylated forms of HSP27, suggesting a role in stress-adaptive signaling. HSP22 contains distinct N- and C-terminal domains that mediate interactions with various protein partners, enabling it to participate in essential cellular processes such as protein quality control, apoptosis, and macroautophagy. Importantly, HSP22 has emerged as a key player in neurodegenerative disease mechanisms. Mutations in the HSPB8 gene are linked to inherited peripheral neuropathies, including distal hereditary motor neuropathy type IIA (dHMNIIA) and Charcot-Marie-Tooth disease type 2L (CMT2L). These disorders are characterized by axonal degeneration and impaired motor function, underscoring HSP22’s role in maintaining neuronal integrity. By promoting the clearance of misfolded proteins and supporting autophagic pathways, HSP22 contributes to cellular resilience in the face of proteotoxic stress—a hallmark of neurodegenerative conditions such as ALS and Alzheimer’s disease. Its dual role in chaperone activity and neuroprotection positions HSP22 as a promising target for therapeutic intervention in neuroscience. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µl. For research use only; not for diagnostic or therapeutic procedures.
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, IHC, ICC/IF, IP
Purification:
Peptide Affinity Purified
Concentration:
1 mg/ml
Reactivity:
Human, Mouse, Rat
Recommended Dilution:
WB (1:1000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~22kDa. Does not cross-react with HSP27 or alpha-crystallin.
Guarantee:
12 months from date of dispatch
Immunogen:
Human HSP22
Immunogen Species:
Human
Accession Number:
NP_055180.1
Gene ID:
26353
Swiss-Prot:
Q9UJY1
Cellular Localization:
Cytoplasm | Nucleus
Alternative Names:
HSPB8, HSP22, Heat shock protein beta-8, Alpha-crystallin C chain, E2-induced gene 1 protein, Protein kinase H11, Small stress protein-like protein, Heat shock protein family B member 8, CRYAC, E2IG1, CMT2L, DHMN2, H11, Heat shock 22kDa protein 8, HMN2, HSB8
References:
1. Kappe G., et al. (2001) Biochem Biophys Acta. 1520: 1-6. 2. Benndorf R., et al. (2001) J Biol Chem. 276: 26753-26761. 3. Sun X., et al. (2004) J Biol Chem. 279: 2394-2402. 4. Kim M.V., et al. (2004) Biochem Biophys Res Commun. 325: 649-652. 5. Wilhelmus M.M., et al. (2006) Acta Neuropathol. (Berl) 111: 139-149.
