Human 10-HETE (10-Hydroxyeicosatetraenoic Acid) ELISA Kit
The Human 10-HETE (10-hydroxyeicosatetraenoic acid) ELISA Kit is designed for the precise quantification of 10-HETE levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.10-HETE is a metabolite of arachidonic acid and has been implicated in inflammation, cell signaling, and the regulation of blood pressure. Its dysregulation has been linked to various pathologies, including cardiovascular disease, cancer, and inflammatory disorders.
As such, measuring 10-HETE levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions. With the Human 10-HETE ELISA Kit, researchers can confidently study the role of 10-HETE in disease mechanisms and explore its potential as a biomarker for monitoring disease progression and treatment efficacy. Trust in the accuracy and reliability of this kit to advance your research in the field of lipid metabolism and inflammatory pathways.
Product Name:
Human 10-HETE (10-Hydroxyeicosatetraenoic Acid) ELISA Kit
SKU:
AEFI00110
Reactivity:
Human
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
0
1
2
3
Serum (n = 10)
85-100%
87-104%
90-101%
EDTA Plasma (n = 10)
86-99%
84-100%
82-98%
Heparin Plasma (n = 10)
82-92%
82-96%
80-95%
Recovery:
0
1
2
Serum (n = 10)
87-104
95
EDTA Plasma (n = 10)
89-100
95
Heparin Plasma (n = 10)
87-102
95
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
