Human 12-LO (Arachidonate 12-Lipoxygenase) CLIA Kit
The Human 12-LO (Arachidonate 12-Lipoxygenase) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of 12-lipoxygenase levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a variety of research applications.12-lipoxygenase is an enzyme that plays a crucial role in the metabolism of arachidonic acid, contributing to the production of various bioactive lipids involved in inflammation and immune responses.
Dysregulation of 12-lipoxygenase has been implicated in various diseases such as cancer, inflammatory disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and high performance, the Human 12-LO CLIA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of 12-lipoxygenase in health and disease.
Product Name:
Human 12-LO (Arachidonate 12-Lipoxygenase) CLIA Kit
SKU:
HUES01277
Target:
Human 12-LO (Arachidonate 12-Lipoxygenase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 12-LO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 12-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 12-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human 12-LO. You can calculate the concentration of Human 12-LO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
86-96
95-112
Average (%)
94
91
103
1:4
Range (%)
89-104
86-97
85-98
Average (%)
96
92
91
1:8
Range (%)
98-115
92-108
91-106
Average (%)
105
100
98
1:16
Range (%)
97-113
83-95
94-109
Average (%)
105
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
96
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.96
500.64
1517.01
213.47
452.44
1571.15
Standard deviation
26.11
54.32
134.26
26.15
50.31
160.57
C V (%)
12.32
10.85
8.85
12.25
11.12
10.22
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human 12-LO concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human 12-LO in samples. No significant cross-reactivity or interference between Human 12-LO and analogues was observed.
