The Human ADP/Acrp30 (Adiponectin) ELISA Kit is expertly designed for the precise quantitative detection of Adiponectin levels in various human biological samples. Adiponectin, a key adipokine, plays a critical role in regulating glucose levels and fatty acid breakdown, influencing insulin sensitivity and inflammation pathways. Its correlation with metabolic disorders, such as obesity and diabetes, underscores its significance in understanding metabolic health. Our ELISA kit ensures exceptional sensitivity and specificity, guaranteeing reliable and reproducible results in your research applications. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for investigating the role of Adiponectin in metabolic pathways and diseases. Trust Assay Genie's ADP/Acrp30 ELISA Kit for accurate quantification and comprehensive analysis of this important biomarker in your studies.
Product Name:
Human ADP/Acrp30 (Adiponectin) ELISA Kit
SKU:
AEES00151
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
