Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit
The Human Acidic Fibroblast Growth Factor 1 (FGF1) ELISA Kit is specifically designed for the precise measurement of FGF1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results, making it the perfect choice for a variety of research purposes.FGF1 is a key growth factor that plays a crucial role in various biological processes, including cell growth, development, and wound healing. Its dysregulation has been linked to several diseases such as cancer, diabetes, and cardiovascular disorders, highlighting its importance as a biomarker for disease detection and prognosis.By using the Human Acidic Fibroblast Growth Factor 1 (FGF1) ELISA Kit, researchers can gain valuable insights into the role of FGF1 in physiological and pathological conditions, paving the way for the development of innovative treatments and therapies.
Product Name:
Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit
SKU:
HUES01358
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
