The Human ALB (Albumin) Quickstep ELISA Kit is designed for the quantitative detection of Human ALB (Albumin) levels in various biological samples. Albumin (ALB) is the most abundant plasma protein, synthesized exclusively by hepatocytes and constituting approximately 60% of total serum protein. It serves critical functions including maintenance of oncotic pressure, transport of hydrophobic molecules (fatty acids, bilirubin, hormones, drugs), buffering of blood pH, and as a major antioxidant. Hypoalbuminemia is a sensitive marker of liver dysfunction, malnutrition, and systemic inflammation, and serum albumin is widely used as a prognostic indicator in liver disease, intensive care, and oncology research. Assay Genie's Human ALB (Albumin) Quickstep ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 18.75 ng/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Human ALB (Albumin) Quickstep ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human ALB (Albumin) Quickstep ELISA Kit
SKU:
QSES072
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
