Human alpha1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit
The Human Alpha-1-Acid Glycoprotein (AGP) ELISA Kit is specifically designed for the accurate and sensitive detection of alpha-1-acid glycoprotein levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results in a variety of research applications.Alpha-1-acid glycoprotein is an important acute phase protein that is involved in various physiological processes, including immune response regulation, drug binding, and inflammation modulation. Abnormal levels of AGP have been linked to certain diseases such as cancer, inflammatory disorders, and infectious diseases, making it a valuable biomarker for diagnostic and research purposes. By using the Human Alpha-1-Acid Glycoprotein ELISA Kit, researchers can gain valuable insights into the role of AGP in health and disease, aiding in the development of potential therapeutic interventions and diagnostic tools. This kit is easy to use and provides accurate measurements, making it an invaluable tool for studying the complexities of alpha-1-acid glycoprotein in human biology.
Product Name:
Human alpha1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit
SKU:
HUES01288
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
