The Human ANG1-7 (Angiotensin 1-7) ELISA Kit is specifically designed for the accurate measurement of Angiotensin 1-7 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Angiotensin 1-7 is a key component of the renin-angiotensin system, playing a critical role in regulating blood pressure and cardiovascular function. Dysregulation of this system has been linked to various cardiovascular and renal diseases.
By accurately measuring Angiotensin 1-7 levels, researchers can gain important insights into these conditions and potentially identify new therapeutic targets.Whether investigating the role of Angiotensin 1-7 in cardiovascular diseases, renal disorders, or other related conditions, the Human ANG1-7 ELISA Kit provides a valuable tool for researchers seeking to advance their understanding of this important biomarker. Trust in the reliability and precision of this ELISA kit for consistent and accurate results in your research endeavors.
Product Name:
Human Ang1-7 (Angiotensin 1-7) ELISA Kit
SKU:
HUES03497
Target:
Human Ang1-7 (Angiotensin 1-7) ELISA Kit
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang1-7. During the reaction, Ang1-7 in the sample or standard competes with a fixed amount of Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang1-7. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang1-7 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
85-95
95-111
Average (%)
91
90
103
1:4
Range (%)
85-96
90-106
98-115
Average (%)
90
97
105
1:8
Range (%)
87-100
87-102
99-113
Average (%)
92
94
106
1:16
Range (%)
89-103
89-104
99-110
Average (%)
96
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
94-104
99
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.1
155.1
482.3
46.2
149.2
518.8
Standard deviation
2.5
9.3
23.6
2.4
6.1
22.3
C V (%)
5.42
6.0
4.89
5.19
4.09
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Ang1-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Ang1-7 in samples. No significant cross-reactivity or interference between Ang1-7 and analogues was observed.
