The Human APOA5 (Apolipoprotein A5) CLIA Kit is specifically designed for the precise measurement of APOA5 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.APOA5 is a key protein that plays a vital role in lipid metabolism and is closely associated with cardiovascular health.
Variations in APOA5 levels have been linked to conditions such as hypertriglyceridemia and cardiovascular diseases, making it a valuable biomarker for studying these disorders and exploring potential therapeutic interventions.With its high performance and reliability, the Human APOA5 CLIA Kit is an indispensable tool for researchers investigating the role of APOA5 in human health and disease.
Product Name:
Human ApoA5 (Apolipoprotein A5) CLIA Kit
SKU:
HUES00342
Target:
Human ApoA5 (Apolipoprotein A5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ApoA5. You can calculate the concentration of Human ApoA5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
101-115
85-98
Average (%)
103
108
91
1:4
Range (%)
103-119
88-100
93-107
Average (%)
110
95
99
1:8
Range (%)
101-115
102-116
87-101
Average (%)
107
108
92
1:16
Range (%)
104-115
96-113
97-114
Average (%)
109
104
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
95-111
102
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.16
143.55
426.29
50.48
156.6
412.39
Standard deviation
6.22
15.75
27.37
4.74
13.56
42.72
C V (%)
11.92
10.97
6.42
9.39
8.66
10.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ApoA5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed.
