Human ARNTL (Aryl hydrocarbon receptor nuclear translocator-like protein 1) ELISA Kit
The Human ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like Protein 1) ELISA Kit is a state-of-the-art tool designed for the precise detection of ARNTL levels in human samples, including serum, plasma, and cell culture supernatants. This ELISA kit is characterized by its high sensitivity and specificity, ensuring dependable and reproducible results for a diverse array of research applications. ARNTL, also known as Bmal1, is a critical component of the circadian clock machinery, playing a pivotal role in regulating the body's internal clock and coordinating various physiological processes with the day-night cycle. Dysregulation of ARNTL has been associated with disruptions in circadian rhythms, which can impact various aspects of health, including metabolism, immune function, and cardiovascular health.
Consequently, ARNTL serves as a valuable biomarker for studying circadian rhythm disorders, metabolic diseases, and other conditions influenced by the body's internal timing mechanisms. By utilizing the Human ARNTL ELISA Kit, researchers can gain valuable insights into the role of ARNTL in health and disease, paving the way for a better understanding of circadian biology and the development of innovative therapeutic strategies targeting circadian rhythm-related disorders.
Product Name:
Human ARNTL (Aryl hydrocarbon receptor nuclear translocator-like protein 1) ELISA Kit
SKU:
AEFI00092
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
90-105%
87-102%
88-99%
EDTA Plasma (n = 5)
82-99%
88-101%
85-100%
Heparin Plasma (n = 5)
81-99%
82-94%
80-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-101
94
EDTA Plasma (n = 5)
85-98
92
Heparin Plasma (n = 5)
87-101
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
