Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
The Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit is specifically designed for the precise measurement of BDNF levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and dependable results, making it well-suited for a wide range of research applications.BDNF is a key neurotrophic factor that plays a crucial role in the growth, survival, and function of neurons in the brain. Dysregulation of BDNF has been associated with various neurological and psychiatric disorders such as depression, Alzheimer's disease, and schizophrenia. Therefore, the detection and quantification of BDNF levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of targeted treatment strategies.Overall, the Human BDNF ELISA Kit from Assaygenie is an essential tool for researchers seeking to study the role of BDNF in neurological disorders and explore potential therapeutic interventions.
Product Name:
Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
SKU:
HUES01297
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
