Human BLC (B-Lymphocyte Chemoattractant) ELISA Kit
The Human BLC (B Lymphocyte Chemoattractant) ELISA Kit is specifically designed for the accurate measurement of BLC levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable tool for various research applications.BLC, also known as CXCL13, plays a pivotal role in immune responses by attracting B lymphocytes to specific sites within the body. Its dysregulation has been implicated in various autoimmune diseases, inflammatory conditions, and lymphoproliferative disorders, making it a key biomarker for studying these conditions and exploring potential therapeutic strategies.With the Human BLC ELISA Kit, researchers can confidently investigate the role of BLC in immune system functioning and disease pathogenesis, paving the way for new insights and advancements in the field of immunology.
Product Name:
Human BLC (B-Lymphocyte Chemoattractant) ELISA Kit
SKU:
HUES01340
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
