The Human CA125 (Carbohydrate Antigen 125) ELISA Kit is specifically designed for the precise measurement of CA125 levels in human serum, plasma, and cell culture supernatants. With its high level of sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for a variety of research purposes.CA125 is a protein that is commonly used as a biomarker for ovarian cancer and other gynecological diseases. It is also known to be elevated in conditions such as endometriosis, uterine fibroids, and pelvic inflammatory disease. The ability to accurately measure CA125 levels can aid in early detection, prognosis, and monitoring of these conditions.Overall, the Human CA125 ELISA Kit is essential for researchers and clinicians looking to study and understand the role of CA125 in disease development and progression, ultimately leading to better diagnostic and therapeutic strategies.
Product Name:
Human CA125 (Carbohydrate Antigen 125) ELISA Kit
SKU:
HUES01799
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 IU/mL
Detection range:
3.13-200 IU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
