Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA kit
The Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA Kit is specifically designed for the precise measurement of CD74 levels in human samples including serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit provides accurate and reproducible results, making it an ideal tool for various research applications.CD74 is a key protein involved in the immune response, specifically in the regulation of antigen presentation. It plays a crucial role in the function of the immune system and has been associated with autoimmune diseases, cancer, and inflammatory disorders. Understanding the levels of CD74 in biological samples can provide valuable insights into these conditions and aid in the development of targeted therapies.This Human CD74 ELISA Kit offers researchers a reliable and efficient method for studying the role of CD74 in various physiological and pathological processes, making it a valuable asset in the field of immunology and related research areas.
Product Name:
Human CD74 (HLA class II histocompatibility antigen gamma chain) ELISA kit
SKU:
HUES03370
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
