Human CGRP (Calcitonin Gene Related Peptide) ELISA Kit
The Human CGRP (Calcitonin Gene-Related Peptide) ELISA Kit is a reliable tool for detecting and measuring levels of CGRP in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results for various research applications.CGRP is a neuropeptide that plays a crucial role in processes like vasodilation, inflammation, and pain transmission. It is implicated in various physiological and pathological conditions, including migraine, neurogenic inflammation, and cardiovascular disorders. By accurately measuring CGRP levels, researchers can better understand the role of this peptide in disease mechanisms and potential therapeutic interventions. Get your Human CGRP ELISA Kit now and unlock new insights into the functions and implications of CGRP in health and disease.
Product Name:
Human CGRP (Calcitonin Gene Related Peptide) ELISA Kit
SKU:
HUES01782
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
