Human CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit
The Human CK-MB (Creatine Kinase-MB Isoenzyme) ELISA Kit is a cutting-edge tool designed for the precise quantification of CK-MB levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are vital for various research applications.CK-MB is an essential enzyme found predominantly in heart muscles, making it a crucial biomarker for diagnosing and monitoring myocardial infarction (heart attack). Elevated levels of CK-MB in the bloodstream indicate damage to the heart muscle, providing valuable insights into cardiac health and potential treatment options. By utilizing the Human CK-MB ELISA Kit, researchers can gain valuable information on cardiac health, improve diagnostic accuracy, and contribute to the development of innovative therapies for heart-related conditions. With its reliable performance and ease of use, this kit is a valuable asset for researchers in the field of cardiology and cardiovascular medicine.
Product Name:
Human CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit
SKU:
HUES02470
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
