The Human CLDN3 (Claudin-3) ELISA Kit offered by Assay Genie is a premier tool for the precise measurement of CLDN3 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for a variety of research endeavors.CLDN3, a member of the claudin family, plays a crucial role in maintaining the integrity of tight junctions between cells and regulating the permeability of epithelial barriers. Dysregulation of CLDN3 has been implicated in various diseases, including cancer and inflammatory disorders, highlighting its significance as a potential biomarker for disease detection and progression.By utilizing the Human CLDN3 ELISA Kit, researchers can gain valuable insight into the role of CLDN3 in health and disease, paving the way for innovative treatment strategies and personalized medicine approaches. Trust Assay Genie for high-quality ELISA kits that drive groundbreaking discoveries in biomedical research.
Product Name:
Human CLDN3 (Claudin 3) ELISA Kit
SKU:
HUES01898
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
