The Human CLDN5 (Claudin-5) ELISA Kit is a reliable tool for measuring levels of CLDN5 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity for accurate and reproducible results, making it suitable for various research applications.CLDN5, a key component of tight junctions, plays a crucial role in maintaining the integrity of the blood-brain barrier and regulating paracellular permeability. Dysregulation of CLDN5 has been implicated in various neurological disorders, including multiple sclerosis, Alzheimer's disease, and stroke. Therefore, accurate measurement of CLDN5 levels is essential for understanding the pathophysiology of these conditions and developing potential therapeutic interventions.By using the Human CLDN5 ELISA Kit, researchers can confidently study the role of CLDN5 in disease progression and explore its potential as a biomarker for diagnosing and monitoring neurological disorders. This kit provides a convenient and efficient platform for investigating the functional significance of CLDN5 in various biological processes.
Product Name:
Human CLDN5 (Claudin 5) ELISA Kit
SKU:
HUES02632
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
