The Human Clusterin ELISA Kit (HUES01325) from Assay Genie is an essential tool for accurately measuring clusterin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results for a variety of research applications.Clusterin, also known as apolipoprotein J, is a versatile protein involved in various physiological and pathological processes, including inflammation, lipid transportation, and cell survival. Its dysregulation has been linked to diseases such as Alzheimer's, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Trust in the Human Clusterin ELISA Kit from Assay Genie to help advance your research efforts and gain valuable insights into the role of clusterin in health and disease. Visit www.assaygenie.com/human-clu-clusterin-elisa-kit-hues01325/ to learn more and order your kit today.
Product Name:
Human CLU (Clusterin) ELISA Kit
SKU:
HUES01325
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
