The Human DEF β2 (Defensin Beta 2) ELISA Kit is specifically designed for the accurate quantification of defensin beta 2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a wide variety of research applications.Defensin beta 2 is a critical antimicrobial peptide that plays a key role in the innate immune system's defense against pathogens. It has been implicated in various inflammatory and infectious diseases, making it a valuable biomarker for studying these conditions and evaluating potential therapeutic interventions.With its advanced technology and reliable performance, the Human DEF β2 (Defensin Beta 2) ELISA Kit is an essential tool for researchers looking to investigate the role of defensin beta 2 in health and disease.
Product Name:
Human DEF beta2/DEFB2 (Defensin Beta 2) ELISA Kit
SKU:
HUES02108
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
85-100
97-112
88-100
Average (%)
91
105
94
1:4
Range (%)
91-103
88-102
83-94
Average (%)
97
93
89
1:8
Range (%)
88-103
85-99
84-95
Average (%)
94
92
88
1:16
Range (%)
93-109
85-98
86-100
Average (%)
100
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
86-97
91
Cell Culture Media (n=8)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
208.96
487.2
1499.38
221.24
532.05
1351.21
Standard deviation
14.25
26.84
85.16
17.08
33.31
117.96
C V (%)
6.82
5.51
5.68
7.72
6.26
8.73
Uniport ID:
O15263
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human DEFβ2/DEFB2 in samples. No significant cross-reactivity or interference between Human DEFβ2/DEFB2 and analogues was observed
