The Human DKK1 (Dickkopf-related protein 1) ELISA Kit is designed for the precise measurement of DKK1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results, making it a valuable tool for various research purposes.DKK1 is a key regulatory protein involved in the Wnt signaling pathway, influencing processes such as cell differentiation, proliferation, and tissue development. Dysregulation of DKK1 has been implicated in various diseases, including cancer, osteoporosis, and Alzheimer's disease, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.With its advanced technology and reliable performance, the Human DKK1 ELISA Kit from Assay Genie provides researchers with a versatile and efficient solution for studying the role of DKK1 in health and disease.
Product Name:
Human DKK1 (Dickkopf Related Protein 1) ELISA Kit
SKU:
HUES01344
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
