The Human DS (Dermatan Sulfate) ELISA Kit. This kit is specifically designed for the precise measurement of dermatan sulfate levels in human samples such as serum, plasma, and cell culture supernatants.With its superior sensitivity and specificity, this ELISA kit ensures accurate and reproducible results, making it an excellent choice for a wide range of research applications. Dermatan sulfate is a key component of the extracellular matrix and is involved in various biological processes including cell adhesion, migration, and growth. Abnormal levels of dermatan sulfate have been linked to conditions such as Ehlers-Danlos syndrome, Marfan syndrome, and other connective tissue disorders. Therefore, this ELISA kit can be a valuable tool for studying these conditions and exploring potential therapeutic targets.Overall, the Human DS (Dermatan Sulfate) ELISA Kit offers researchers a reliable and efficient method for quantifying dermatan sulfate levels in human samples, providing valuable insights into the role of this molecule in health and disease.
Product Name:
Human DS (Dermatan Sulfate) ELISA Kit
SKU:
HUES02713
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
