Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit
The Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit is specifically designed for the precise measurement of EGFR levels in human serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes. EGFR is a key receptor protein that plays a vital role in cell growth and division, as well as cell survival. Dysregulation of EGFR has been implicated in various diseases, including cancer and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing therapeutic interventions. With the Human EGFR ELISA Kit, researchers can confidently investigate the role of EGFR in disease progression and treatment response, advancing our understanding of pathophysiological mechanisms and paving the way for targeted therapeutic strategies. Order your kit today and unlock the potential of EGFR research.
Product Name:
Human EGFR (Epidermal Growth Factor Receptor) ELISA Kit
SKU:
HUES01347
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
