Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
The Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit is meticulously crafted to enable the quantitative detection of FABP1 levels in various biological samples of human origin. Fatty Acid Binding Protein 1, primarily expressed in the liver, is involved in intracellular fatty acid transport and metabolism, playing a crucial role in lipid homeostasis and metabolic processes. Accurate measurement of FABP1 can provide insights into lipid metabolism, liver function, and metabolic disorders, making it a valuable tool for research in liver-related pathologies. Our ELISA kit offers outstanding sensitivity and specificity, ensuring precise and reproducible results essential for studying FABP1's function and diagnostic potential. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for cutting-edge research applications. Trust Assay Genie's FABP1 ELISA Kit for accurate and dependable quantification of this vital biomarker in your investigations.
Product Name:
Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit
SKU:
AEES00161
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
