Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
The Human FABP4 (Fatty Acid Binding Protein 4) ELISA Kit is designed for the accurate detection of FABP4 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.FABP4 is a key protein involved in fatty acid metabolism and adipocyte function. Its levels have been implicated in various metabolic disorders such as obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential treatment options. Overall, the Human FABP4 ELISA Kit provides researchers with a powerful tool for studying the role of FABP4 in metabolic and cardiovascular diseases, allowing for precise and accurate measurements of FABP4 levels in biological samples.
Product Name:
Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
SKU:
HUES01520
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
