The Human FBN1 (Fibrillin-1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of Fibrillin-1 levels in human samples, including serum, plasma, and cell culture supernatants. Fibrillin-1 is a key component of extracellular matrix microfibrils and plays a critical role in maintaining tissue structure and integrity.This kit offers accurate and reliable results, making it an excellent tool for researchers studying conditions such as Marfan syndrome, a genetic disorder characterized by mutations in the FBN1 gene. By measuring Fibrillin-1 levels, this kit can provide valuable insights into the pathogenesis of Marfan syndrome and other related disorders, paving the way for the development of potential diagnostic and therapeutic strategies.Overall, the Human FBN1 (Fibrillin-1) CLIA Kit is a valuable resource for research aimed at understanding the role of Fibrillin-1 in health and disease, offering researchers a powerful tool to advance their studies in this important field.
Product Name:
Human FBN1 (Fibrillin 1) CLIA Kit
SKU:
HUES01183
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
