The Human FPN (Ferroportin) ELISA Kit is specifically designed for the precise measurement of ferroportin levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research applications.Ferroportin is a key protein involved in iron metabolism, responsible for transporting iron out of cells and into the bloodstream. Dysregulation of ferroportin has been linked to conditions like iron overload disorders and anemia, highlighting its importance as a potential biomarker for studying these diseases and developing targeted therapies.With the Human FPN ELISA Kit, researchers can confidently investigate the role of ferroportin in various physiological and pathological processes, paving the way for deeper insights into iron homeostasis and related disorders.
Product Name:
Human FPN (Ferroportin) ELISA Kit
SKU:
HUES03226
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
