The Human FSCN (Fascin) CLIA Kit is a cutting-edge tool designed for the precise measurement of Fascin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, assisting researchers in obtaining accurate and consistent results for a variety of research applications.Fascin is a key protein that plays a critical role in cell migration and the formation of filopodia, which are essential for various cellular processes such as cell adhesion and motility.
Dysregulation of Fascin has been linked to various diseases including cancer, where it promotes tumor metastasis and invasion. Understanding the role of Fascin is crucial in unraveling the mechanisms underlying these diseases and developing targeted therapies.Overall, the Human FSCN (Fascin) CLIA Kit is a valuable tool for researchers looking to study the functions of Fascin and its implications in disease pathology, ultimately leading to potential therapeutic interventions.
Product Name:
Human FSCN (Fascin) CLIA Kit
SKU:
HUES00809
Target:
Human FSCN (Fascin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FSCN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FSCN. You can calculate the concentration of Human FSCN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
96-110
94-108
Average (%)
107
101
101
1:4
Range (%)
99-115
90-105
87-98
Average (%)
107
96
92
1:8
Range (%)
102-117
97-113
97-111
Average (%)
109
103
103
1:16
Range (%)
98-116
84-98
96-112
Average (%)
106
90
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.07
4.62
0.52
0.97
4.68
Standard deviation
0.07
0.09
0.43
0.06
0.09
0.45
C V (%)
13.46
8.41
9.31
11.54
9.28
9.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FSCN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FSCN in samples. No significant cross-reactivity or interference between Human FSCN and analogues was observed.
