The Human FXN (Frataxin) ELISA Kit is specifically designed for the precise measurement of frataxin levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are essential for various research applications.Frataxin is a key protein involved in regulating iron metabolism and mitochondrial function, making it critical for overall cellular health. Dysregulation of frataxin levels has been linked to various diseases, including Friedreich's ataxia, a neurodegenerative disorder characterized by progressive muscle weakness and lack of coordination. By accurately quantifying frataxin levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify new therapeutic targets. The Human FXN ELISA Kit is a valuable tool for studying frataxin-related conditions and advancing the development of treatments for these disorders.
Product Name:
Human FXN (Frataxin) ELISA Kit
SKU:
HUES02636
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
14.06 pg/mL
Detection range:
23.44-1500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
