The Human Gal1 (Galectin-1) ELISA Kit is specifically designed to detect Galectin-1 levels in human serum, plasma, and cell culture supernatants with high sensitivity and specificity. This reliable and reproducible kit is ideal for a variety of research applications.Galectin-1 is a key protein involved in cell adhesion, cell activation, and modulation of immune responses. It plays a crucial role in various physiological and pathological processes, including cancer, inflammation, and autoimmune diseases. Therefore, this ELISA kit is essential for studying these conditions and developing potential therapies.With easy-to-follow instructions and quick assay procedures, the Human Gal1 (Galectin-1) ELISA Kit from Assaygenie is a valuable tool for researchers looking to accurately measure Galectin-1 levels and further understand its biological functions.
Product Name:
Human GAL1 (Galectin 1) ELISA Kit
SKU:
HUES02150
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
