The Human Gal3 (Galectin-3) CLIA Kit is designed for the precise measurement of Gal3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.Galectin-3, also known as Gal3, is a multifunctional protein involved in cell adhesion, cell proliferation, and inflammation. High levels of Gal3 have been linked to various diseases, including cancer, heart failure, and fibrosis, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
With its reliable performance and user-friendly design, the Human Gal3 CLIA Kit is an invaluable tool for researchers investigating the role of Gal3 in disease pathogenesis and therapeutic development.
Product Name:
Human GAL3 (Galectin 3) CLIA Kit
SKU:
HUES00839
Target:
Human GAL3 (Galectin 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL3. You can calculate the concentration of Human GAL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
101-114
88-100
Average (%)
106
107
93
1:4
Range (%)
89-104
91-106
101-117
Average (%)
96
98
107
1:8
Range (%)
89-103
93-106
92-103
Average (%)
94
101
97
1:16
Range (%)
101-116
93-110
101-117
Average (%)
107
100
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
101
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.64
302.32
798.06
110.52
316.19
728.1
Standard deviation
11.3
25.15
72.46
12.88
30.7
74.41
C V (%)
10.8
8.32
9.08
11.65
9.71
10.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
