Human GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
The Human GDNF (Glial Cell Line-Derived Neurotrophic Factor) ELISA Kit is a powerful tool for detecting GDNF levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it ideal for a variety of research applications.GDNF is a key protein involved in promoting the survival and growth of neurons, particularly dopaminergic neurons in the brain. Its role in neuroprotection and neuroregeneration makes it an important target for studying neurodegenerative diseases such as Parkinson's disease and amyotrophic lateral sclerosis (ALS). By measuring GDNF levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic strategies.Overall, the Human GDNF ELISA Kit from AssayGenie is a valuable tool for studying the role of GDNF in neurobiology and developing novel treatments for neurological disorders. Order yours today and take your research to the next level.
Product Name:
Human GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
SKU:
HUES02517
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
