Human GRO beta (Growth Regulated Oncogene Beta) ELISA Kit
The Human Gro-beta (Growth Regulated Oncogene Beta) ELISA Kit from Assay Genie is specifically designed for the accurate measurement of Gro-beta levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Gro-beta, also known as CXCL2, is a key regulator of inflammation and immune response. It plays a crucial role in mediating the recruitment of neutrophils to sites of infection or injury, as well as promoting tumor growth and metastasis. Detecting Gro-beta levels can provide valuable insights into the pathogenesis of various diseases, including cancer, inflammatory disorders, and autoimmune conditions.By utilizing the Human Gro-beta ELISA Kit from Assay Genie, researchers can gain a better understanding of Gro-beta's role in health and disease, paving the way for potential therapeutic interventions and improved patient outcomes.
Product Name:
Human GRO beta (Growth Regulated Oncogene Beta) ELISA Kit
SKU:
HUES02851
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.