Human GRP78 (Glucose Regulated Protein 78) ELISA Kit
The Human GRP78 (Glucose-Regulated Protein 78) ELISA Kit is specifically designed for the precise and reliable measurement of GRP78 levels in human biological samples such as serum, plasma, and cell culture supernatants. GRP78, also known as BiP, is a key molecular chaperone involved in protein folding and assembly, particularly in response to cellular stress such as glucose deprivation or endoplasmic reticulum (ER) stress. Abnormal expression of GRP78 has been linked to various diseases including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for research and diagnostic purposes. This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for studying the role of GRP78 in disease pathogenesis and potential therapeutic interventions. Ideal for a wide range of research applications, the Human GRP78 ELISA Kit provides a valuable tool for investigating the molecular mechanisms underlying various health conditions.
Product Name:
Human GRP78 (Glucose Regulated Protein 78) ELISA Kit
SKU:
HUES03558
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
