The Human HAase (Hyaluronidase) ELISA Kit is specifically designed for the precise measurement of hyaluronidase levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research purposes. Hyaluronidase is an enzyme known for its role in breaking down hyaluronic acid, a key component of the extracellular matrix. This enzyme is involved in numerous biological processes such as tissue remodeling, inflammation, and wound healing. Its dysregulation has been linked to various diseases including cancer, arthritis, and skin disorders, highlighting its importance as a biomarker in studying these conditions and potentially developing therapeutic interventions. The Human HAase ELISA Kit provides researchers with a reliable tool to investigate the levels of hyaluronidase in biological samples, offering valuable insights into its physiological and pathological roles. Don't miss out on the opportunity to enhance your research with this cutting-edge ELISA kit from AssayGenie.
Product Name:
Human HAase (Hyaluronidase) ELISA Kit
SKU:
HUES03096
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
