The Human HE4 (epididymal protein 4) ELISA Kit is a specialized assay intended for the precise quantification of HE4 levels in human biological samples. HE4, also known as a tumor marker, is a glycoprotein that is involved in various biological functions and is predominantly expressed in the male reproductive tract and female genital organs. Elevated levels of HE4 have been linked to gynecologic cancers, making it a valuable biomarker for the early detection and monitoring of ovarian and endometrial cancers. This ELISA kit offers a reliable platform for researchers and clinicians to accurately measure HE4 levels in serum or plasma samples, facilitating investigations related to cancer diagnostics, prognosis, and therapeutic monitoring. By detecting changes in HE4 expression, researchers can gain insights into disease progression, response to treatment, and overall patient management. The Human HE4 ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and ease of use, making it an excellent choice for research studies focusing on cancer biomarkers, tumor detection, and personalized medicine approaches.
Product Name:
Human HE4 (epididymal protein 4) ELISA Kit
SKU:
AEES00656
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
14.13 pg/mL
Detection range:
39.06-2500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
