The Human HSP-90 (Heat Shock Protein 90) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of HSP-90 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for a variety of research applications.HSP-90 is a molecular chaperone protein that plays a critical role in protein folding, stability, and regulation of client proteins. It is involved in various cellular processes and is often upregulated in response to cellular stress, making it a key player in maintaining cellular homeostasis. Studying HSP-90 levels can provide valuable insights into cellular stress responses, protein quality control mechanisms, and the development of therapeutic strategies for diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. The Human HSP-90 CLIA Kit is an invaluable tool for researchers looking to investigate the role of HSP-90 in health and disease.
Product Name:
Human HSP-90 (Heat Shock Protein 90) CLIA Kit
SKU:
HUES01031
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
