Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit
The Human I-TAC (Interferon-Inducible T Cell Alpha Chemoattractant) ELISA Kit is specifically designed for the precise detection of I-TAC levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results, making it well-suited for various research applications.I-TAC is a key chemokine involved in immune response regulation, specifically in attracting and activating T cells. It plays a crucial role in inflammatory processes and immune cell recruitment to sites of infection or injury. The measurement of I-TAC levels is essential for studying immune responses, inflammatory diseases, and potential therapeutic interventions.Overall, the Human I-TAC ELISA Kit provides researchers with a valuable tool for investigating the role of I-TAC in immune regulation and inflammatory processes, ultimately contributing to advancements in the understanding and treatment of various immune-related conditions.
Product Name:
Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit
SKU:
HUES01338
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
90-104
94-108
91-103
Average (%)
95
99
97
1:4
Range (%)
86-101
79-93
88-101
Average (%)
92
85
93
1:8
Range (%)
88-99
85-100
88-104
Average (%)
93
92
95
1:16
Range (%)
88-102
84-95
82-97
Average (%)
96
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
94-107
101
Cell Culture Media (n=8)
97-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.11
423.7
1895.25
198.12
435.7
1829.1
Standard deviation
10.57
17.46
87.94
14.26
34.38
120.9
C V (%)
5.13
4.12
4.64
7.2
7.89
6.61
Uniport ID:
O14625
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human I-TAC/CXCL11 in samples. No significant cross-reactivity or interference between Human I-TAC/CXCL11 and analogues was observed
