The Human IDO (Indoleamine 2,3-Dioxygenase) ELISA Kit is specifically designed for the accurate measurement of IDO levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.IDO is a key enzyme involved in the regulation of immune responses and inflammation by catalyzing the breakdown of the amino acid tryptophan. Dysregulation of IDO activity has been implicated in various diseases, including cancer, infectious diseases, and autoimmune disorders, highlighting its importance as a potential diagnostic and therapeutic target.
With its robust performance and usability, the Human IDO ELISA Kit is a valuable tool for researchers studying the role of IDO in disease pathogenesis and exploring novel treatment strategies. It provides a convenient and effective means of quantifying IDO levels in biological samples, facilitating insights into its physiological and pathological functions.
Product Name:
Human IDO (Indoleamine-2,3-Dioxygenase) ELISA Kit
SKU:
HUES03063
Target:
Human IDO (Indoleamine-2,3-Dioxygenase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IDO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IDO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IDO. You can calculate the concentration of Human IDO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
100-111
84-96
Average (%)
91
105
91
1:4
Range (%)
90-103
84-97
84-96
Average (%)
96
90
91
1:8
Range (%)
86-101
82-94
82-97
Average (%)
93
89
89
1:16
Range (%)
89-99
81-95
84-95
Average (%)
94
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
92-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
1.81
7.37
1.03
1.96
7.88
Standard deviation
0.06
0.1
0.23
0.07
0.12
0.41
C V (%)
6.32
5.52
3.12
6.8
6.12
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IDO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IDO in samples. No significant cross-reactivity or interference between Human IDO and analogues was observed.
