The Human IDO (Indoleamine 2,3-Dioxygenase) ELISA Kit is specifically designed for the accurate measurement of IDO levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.IDO is a key enzyme involved in the regulation of immune responses and inflammation by catalyzing the breakdown of the amino acid tryptophan. Dysregulation of IDO activity has been implicated in various diseases, including cancer, infectious diseases, and autoimmune disorders, highlighting its importance as a potential diagnostic and therapeutic target. With its robust performance and usability, the Human IDO ELISA Kit is a valuable tool for researchers studying the role of IDO in disease pathogenesis and exploring novel treatment strategies. It provides a convenient and effective means of quantifying IDO levels in biological samples, facilitating insights into its physiological and pathological functions.
Product Name:
Human IDO (Indoleamine-2,3-Dioxygenase) ELISA Kit
SKU:
HUES03063
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
