The Human IL-15 ELISA Kit is specially designed for the precise measurement of Interleukin-15 (IL-15) levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results, making it an indispensable tool for a variety of research applications.IL-15 is a key cytokine that plays a crucial role in the regulation of immune responses, particularly in the development and function of natural killer (NK) cells and CD8+ T cells. Dysregulation of IL-15 has been linked to various autoimmune diseases, inflammatory disorders, and cancer, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions. With its user-friendly protocol and high-performance capabilities, the Human IL-15 ELISA Kit from Assay Genie provides researchers with a reliable and accurate solution for studying the role of IL-15 in health and disease. Unlock new insights and advance your research with this innovative ELISA kit.
Product Name:
Human IL-15 ELISA Kit
SKU:
HUES01475
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
