The Human IL-4 ELISA Kit is a powerful tool for detecting and measuring interleukin-4 levels in human samples. IL-4 is a key cytokine involved in immune responses, inflammation, and allergic reactions. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in serum, plasma, and cell culture supernatants.IL-4 plays a crucial role in regulating the immune system and can be implicated in various diseases and disorders, including asthma, allergic reactions, and autoimmune diseases. Studying IL-4 levels can provide valuable insights into the underlying mechanisms of these conditions and aid in the development of targeted therapies.With its advanced technology and easy-to-use format, the Human IL-4 ELISA Kit is an essential tool for researchers and clinicians looking to explore the role of IL-4 in health and disease. Order now and take your research to the next level with this cutting-edge kit from Assay Genie.
Product Name:
Human IL-4 ELISA Kit
SKU:
HUES01387
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
