The Human IL-5 (Interleukin 5) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of IL-5 levels in human serum, plasma, and cell culture supernatants. This kit ensures accurate and reproducible results, making it suitable for a wide range of research applications.IL-5 is a key cytokine involved in the regulation of immune responses, specifically in the development and activation of eosinophils. Dysregulation of IL-5 is associated with allergic diseases, asthma, and various inflammatory conditions. As such, measuring IL-5 levels can provide valuable insights into the underlying mechanisms of these diseases and help in the development of targeted therapies.The Human IL-5 ELISA Kit from Assay Genie offers researchers a reliable tool for studying the role of IL-5 in various pathological conditions and evaluating the efficacy of potential treatments. With its high sensitivity and specificity, this kit is an essential resource for advancing our understanding of immune-mediated diseases.
Product Name:
Human IL-5 ELISA Kit
SKU:
HUES01458
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
