The Human IL-6 CLIA Kit is a highly sensitive and specific assay designed for the quantification of Interleukin-6 (IL-6) levels in human samples such as serum, plasma, and cell culture supernatants. IL-6 is a key cytokine involved in the immune response and inflammation, playing a crucial role in various diseases and conditions.This CLIA kit offers reliable and reproducible results, making it suitable for a wide range of research applications in the fields of immunology, oncology, and infectious diseases.
By accurately measuring IL-6 levels, researchers can gain valuable insights into the pathophysiology of various diseases and potentially identify novel therapeutic targets.With its high sensitivity and specificity, the Human IL-6 CLIA Kit is a valuable tool for researchers looking to explore the role of IL-6 in health and disease. Order yours today to advance your research efforts and uncover new possibilities in the field of cytokine biology.
Product Name:
Human IL-6 CLIA Kit
SKU:
HUES00098
Target:
Human IL-6
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
93-104
98-113
Average (%)
95
99
105
1:4
Range (%)
104-120
101-118
96-109
Average (%)
110
109
104
1:8
Range (%)
101-115
103-117
102-114
Average (%)
108
110
107
1:16
Range (%)
92-106
88-102
90-100
Average (%)
100
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
94-111
102
Cell culture media (n=5)
98-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.64
91.69
379.68
36.94
88.53
350.22
Standard deviation
3.59
10.67
31.02
3.8
6.59
34.15
C V (%)
9.54
11.64
8.17
10.29
7.44
9.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.
