Human Interferon Beta (IFNb) ELISA Kit



ELISA Kit Technical Manual

Human Interferon Beta (IFNb) ELISA Kit

Product NameHuman Interferon Beta (IFNb) ELISA Kit
SynonymsIFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast
Detection range7.81-500pg/mL
Size96 assays
Shelf Life12 months
RecoveryMatrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Recovery Table
MatrixRecovery range (%)Average(%)
serum(n=5) 80-10291
EDTA plasma(n=5) 81-100 90
heparin plasma(n=5) 80-89 84
LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Linearity Table
EDTA plasma(n=5)88-101%86-95%90-102%80-93%
heparin plasma(n=5)80-91%82-90%95-104%79-95%
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: : CV<12%
StabilityThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Human Interferon Beta (IFNb) ELISA Kit Assay Summary
  • 1. Prepare all reagents, samples and standards.
  • 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃.
  • 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃.
  • 4. Aspirate and wash 3 times.
  • 5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃.
  • 6. Aspirate and wash 5 times.
  • 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃.
  • 8.Add 50µL Stop Solution. Read at 450nm immediately.
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